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These fish (n = 8; 1.35 ± 0.74 kg, 46.70 ± 0.79 cm in total length; mean ± S.E.M.) were implanted with a centi-HRT ACT tag and returned to their tank to recover for 5 days. On the 6th day at 12:00 p.m., the pre-programmed centi-HRT ACT tags began saving and recording ECGs/fH (at 100 Hz for 6 s), tri-axial acceleration (at 1 Hz for 60 s) and temperature every 2 min. At 1:00 p.m., the fish were netted, lightly anaesthetized in seawater containing 0.1 g L−1 MS-222 and transferred to an 81 L Blazka-type swim tunnel (25 cm diam., swimming section 120 cm in length). The swim tunnel was maintained at ~ 11 °C by a temperature controlled, and aerated, external reservoir and set to a low current velocity (~ 0.2 body lengths s−1, BL s−1; ~ 10 cm s−1). This allowed the fish to rest on the bottom of the swim tunnel and maintain an upright position without swimming. The front of the swim tunnel was covered with black plastic to encourage the fish to seek refuge near the front of the tunnel.

Prystay TS, Eliason EJ, Lawrence MJ, Dick M, Brownscombe JW, Patterson DA, Crossin GT, Hinch SG, Cooke SJ. The influence of water temperature on sockeye salmon heart rate recovery following simulated fisheries interactions. Conserv Physiol. 2017;5(1):1–12.

Wallerius ML, Grӓns A, Koeck B, Berger D, Sandblom E, Ekstrom A, Arlinghaus R, Johnsson JI. Socially induced stress and behavioural inhibition in response to angling exposure in rainbow trout. Fish Manag Ecol. 2019;00:1–10.

This paper evaluated the validity and reliability of centi-HRT ACT tags for recording the physiology and behavior of free-swimming Atlantic salmon (Salmo salar) by addressing four questions. First, do the acceleration parameters calculated by the Star-Oddi algorithm provide meaningful estimates of swimming speed and tail beat frequency, and information on swimming behavior? Second, how long does it take fish to recover from the effects of surgery? Third, can the tags record subtle changes in fish physiology and behavior (e.g., diel patterns)? Finally, over long periods of time (i.e., weeks to months), does the initial tag placement/orientation change and do these tags continue to accurately record fH and acceleration?